Abnormal Circulating Levels of Matrix Metalloproteinases and their Inhibitors in Diabetes Mellitus

作者：Zayani Yosra; Allal Elasmi Monia^*; Jacob Marie Paule; Zidi Wiem; Ftouhi Bochra; Feki Moncef; Slimane Hedia; Kaabachi Naziha

来源：Clinical Laboratory, 2012, 58(7-8): 779-785.

DOI：10.7754/Clin.Lab.2011.110904

• Matrix metalloproteinases
• tissue inhibitors Matrix metalloproteinases
• Type 2 diabetes
• gelatin zymography
• ELISA

Background: The aim of this study was to determine plasma levels of matrix metalloproteinases (MMPs) 2, 3, and 9 and their tissue inhibitors (TIMPs) 1 and 2 in type 2 diabetic patients (T2DM) compared to healthy subjects. %26lt;br%26gt;Methods: The study involved 54 patients with T2DM and 57 age and gender matched healthy adults as controls. MMPs 2 and 9 were analyzed by gelatin zymography and MMP-3 and TIMPs 1 and 2 by ELISA. %26lt;br%26gt;Results: For technical feasibility, MMPs 2 and 9 were expressed in pro forms. Pro-MMP-9 was significantly higher (p%26lt;0.05), whereas TIMP-1 and TIMP-2 levels were significantly decreased (p%26lt;0.01) in patients with T2DM compared to controls. The MMP-3/TIMP-1 and the MMP-3/TMP-2 ratios were significantly higher in T2DM patients than controls (p%26lt;0.05). Fasting plasma glucose was inversely correlated with TIMP-1 (r = -0.412, p%26lt;0.01) and TIMP-2 (r = -0.315, p%26lt;0.001), but was not associated with MMPs. %26lt;br%26gt;Conclusions: The present study identified abnormalities in plasma markers for extracellular matrix metabolism in T2DM. The new parameters would constitute an effective approach to explore the complications of uncontrolled diabetes. (Clin. Lab. 2012;58:779-785. DOI: 10.7754/Clin.Lab.2011.110904)

• 出版日期2012