Anti-baculovirus activity in a protein extracted from the exoskeleton of Pleuroncodes planipes [Decapoda: Galatheidae]

作者：Galvez Romero G; Salas Rojas M; Mendoza Hernandez G; Blanco Favela F; Aguilar Setien A^*

来源：Fish & Shellfish Immunology, 2011, 31(3): 482-484.

DOI：10.1016/j.fsi.2011.06.007

• Antiviral activity
• Metalloprotein
• Pleuroncodes planipes
• Exoskeleton

Antiviral activity (99.5% inhibition) against the Autographa cahfornica polyhedrosis nuclear virus AcNPV+GFP was shown by a polypeptide of approximately 10 kDa, isolated from the exoskeleton of Pleuroncodes planipes, the pelagic red crab. This thermo-stable polypeptide retained its anti-viral properties after being exposed to 76 degrees C for 30 min and showed no apparent cytotoxic effect. Its anti-viral activity was observed when incubated with the virus, previous to the inoculation of cells. Using Tandem Mass Spectrometry (LC/ESI-MS/MS), this polypeptide showed sequence identity to a fragment of a myohemeritrin-like metalloprotein found in the Scoloplos armiger sea worm (VFYANLDEEHK).

• 出版日期2011-9