A simplified and cost-effective method combining real-time PCR and pyrosequencing for detection of aac(6 %26apos;)-Ib-cr gene

作者：Guillard Thomas^*; Fontaine Nicolas; Limelette Anne; Lebreil Anne Laure; Madoux Janick; de Champs Christophe

来源：Journal of Microbiological Methods, 2013, 95(2): 268-271.

DOI：10.1016/j.mimet.2013.09.015

• Real-time PCR
• Pyrosequencing
• Plasmid-mediated quinolone resistance aac(6 %26apos
• )-Ib-cr
• SYBR Green

We previously described a pyrosequencing-based method able to guarantee detection of aac(6%26apos;)-Ib-cr by characterizing precisely the single nucleotide polymorphisms leading to Trp102Arg and Asp179Tyr. By gaining in simplicity and cost-effectiveness, through the use of real-time PCR, we propose here a cutting-edge evolution of our existing pyrosequencing method.

• 出版日期2013-11