A rapid LC-MS/MS quantification of peramivir using a simple and inexpensive sample precipitation: application to PK

作者：Wang, Zhan-Zhang; Zhang, Ming; Shang, De-Wei; Ni, Xiao-Jia; Hu, Jin-Qing; Qiu, Chang; Wen, Yu-Guan^*

来源：Bioanalysis, 2015, 7(3): 319-332.

DOI：10.4155/bio.14.248

• TANDEM MASS-SPECTROMETRY
• HUMAN PLASMA
• INTRAVENOUS PERAMIVIR
• ANTIINFLUENZA DRUG
• LIQUID
• OSELTAMIVIR
• PHARMACOKINETICS
• THROUGHPUT
• PEPTIDES
• EFFICACY

Aim: Peramivir is a newly approved selective neuraminidase inhibitor designed to inhibit influenza virus infection. Methodology/results: We report a robust and sensitive method utilizing simple precipitation extraction with LC-MS/MS for the high-throughput quantification. Addition of 0.06 M of ammonium formate and 0.1% formic acid in mobile phase could help reduce the matrix effect. This method uses 100 mu l of plasma and covers a linear concentration range from 5 to 10,000 ng/ml. Other validation parameters are also evaluated and meet regulatory expectations by US FDA guidelines. Conclusion: The developed HPLC-MS/MS method has been successfully applied to support a clinical pharmacokinetic study. The strategy presented here can be applied elsewhere and may be useful for other amphiphilic drugs.

• 出版日期2015
• 单位广州医科大学