An Efficient Plant Regeneration System via Direct Organogenesis with in Vitro Flavonoid Accumulation and Analysis of Genetic Fidelity among Regenerants of Teucrium polium L.

作者：Rad Faraz Arash^*; Jafari Morad; Khezrinejad Nabi; Miandoab Mahmoud Pouryousef

来源：Horticulture Environment and Biotechnology, 2014, 55(6): 568-577.

DOI：10.1007/s13580-014-0611-7

• adventitious shoot formation
• flavonoid content
• in vitro propagation
• seed pretreatment

An efficient and rapid plant regeneration system via direct organogenesis was established for felty germander (Teucrium polium L.), an endangered and valuable medicinal plant. Induction of shoot organogenesis was obtained using nodal explants and preconditioned intact seedlings on the MS medium supplemented with various concentrations and combinations of plant growth regulators. The highest number of shoots (7.14/explants) was obtained from nodal explants cultured on MS containing 2.0 mg.L-1 BAP and 0.5 mg.L-1 NAA. For preconditioning, Best response in terms of number of adventitious shoots per explant (6.85) and greatest shoot length (5.43 cm) was obtained with seedlings perpetrated in 0.5 mg.L-1 TDZ. The rooted plantlets were acclimatized successfully in the greenhouse with a survival rate of 100%. Significant differences (p < 0.05) were found between the greenhouse-grown tissue culture raised plants in total flavonoid contents. Methanolic extracts of plants raised on TDZ (0.5 mg.L-1) containing medium showed the highest amount of flavonoid contents (1.67 mg Ru.g(-1) DW) and a higher antioxidant activity (52.31%) in comparison with the field-grown mother plant. Randomly amplified polymorphic DNA (RAPD) analysis of plants raised preconditioning procedure revealed there were no somaclonal variations among them. Our results indicated that the cytokinin-preconditioning mediated micropropagation protocol could be used for the production of a large number of true-to-type plants of T. polium, which may contain higher quantities of valuable phytochemicals than those of wild plants.

• 出版日期2014-12