Aflatoxin B-1 interferes with the antigen-presenting capacity of porcine dendritic cells

作者：Mehrzad Jalil; Devriendt Bert; Baert Kim; Cox Eric^*

来源：Toxicology in Vitro, 2014, 28(4): 531-537.

DOI：10.1016/j.tiv.2013.11.015

• Aflatoxins
• Cytokines
• Dendritic cells
• T cells
• Porcine
• Immunotoxicity

Aflatoxins (AFs) are harmful to animal and human health upon consumption of AF-contaminated feed or food. Among many forms of AFs, aflatoxin B-1 (AFB(1)) is the most toxic and carcinogenic. In addition, AFB(1) impairs cell-mediated immunity, although the exact mechanism of this immunotoxicity is currently unknown. By far the most pivotal cells in the induction of immune responses are dendritic cells (DCs). These highly specialised cells dictate T-cell polarisation depending on the nature of the encountered antigens and environmental cues. To elucidate the effect of AFB(1) on the function of DCs, we used porcine monocyte-derived DCs (MoDCs) as a model system. A low dose of AFB(1) transiently reduced the phagocytic capacity of MoDCs. Furthermore, as compared to untreated MoDCs, AFB(1) significantly downregulated the cell surface expression of the co-stimulatory molecule CD40 at 12 h post treatment, while at 24 h the membrane expression levels of CD40 and the activation marker CD25 were significantly upregulated. Interestingly, the T-cell proliferation-inducing capacity of DCs was diminished upon AFB(1) treatment. In contrast, the cytokine secretion pattern of AFB-rtreated MoDCs was similar to mock-treated MoDCs. The results in this study indicate that a low level of AFB(1) dysregulates the antigen-presenting capacity of DCs, which could explain the observed immunotoxicity of this mycotoxin, and further stress the need to reduce AFB(1) levels in agricultural commodities.

• 出版日期2014-6