A new method for antimicrobial susceptibility testing of vitro-cultured bacteria by means of resonance light scattering technique

作者：Shi, Yu Jun^*; Chen, Jun; Xu, Ming

来源：Journal of Microbiology and Biotechnology, 2008, 18(1): 118-123.

• MTT
• antimicrobial susceptibility testing
• resonance light scattering

A new method for antimicrobial susceptibility testing of vitro-cultured bacteria on an ordinary fluorescence spectrometer was developed. The viable bacteria reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MITT) to produce insoluble particles that displayed intense resonance scattering light. The assay showed a linear relationship between the number of viable bacteria and the intensity of resonance scattering light. Dead bacteria were unable to reduce MTT. Methicillin-resistant Staphylococcus aureus exposed to flavonoids from Marchantia convoluta showed a flavonoids concentration-dependent inhibition of the ability to reduce MITT. In the assay, less than 12 h was required to attain susceptibility results and fewer bacteria were utilized than in traditional methods. The RLS technique could, in combination with the MTT assay, be a rapid and sensitive measuring method to determine the in vitro activity of new antimicrobials.

• 出版日期2008-1
• 单位南通大学; 中国人民解放军镇江船艇学院