Antioxidant Activity of Protein Hydrolysates from Aqueous Extract of Velvet Antler (Cervus elaphus) as Influenced by Molecular Weight and Enzymes

作者：Zhao, Lei; Luo, Yang-Chao; Wang, Cheng-Tao; Ji, Bao-Ping^*

来源：Natural Product Communications, 2011, 6(11): 1683-1688.

• Velvet antler
• antioxidant
• ultrafiltration
• protein hydrolysates
• peptide fractions

The crude protein hydrolysates from aqueous extract of velvet antler (AEVA) were prepared by simulated gastrointestinal digestion (SGI, pepsin-pancreatin) using pancreatin-pepsin, alcalase and neutrase. The resulting hydrolysates were separated by sequential ultrafiltration into four fractions. The antioxidant activities of peptide fractions were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging and Fe2+-chelating assays. Results showed that the hydrolysate prepared by SGI had a low degree of hydrolysis, which was significantly improved with altered proteases, such as pancreatin-pepsin and alcalase. Antioxidant activities of peptide fractions varied with molecular weight (MW) and the enzyme used. Generally, low-MW peptide fractions had higher ABTS radical scavenging activity and Fe2+-chelating ability, and high-MW peptide fractions were more effective in DPPH radical scavenging activity and reducing power.

• 出版日期2011-11
• 单位北京工商大学; 中国农业大学