Comparison of a two-stage and a combined single stage salt-acid based lignocellulosic pretreatment for enhancing enzymatic saccharification

作者：Moodley Preshanthan; Kana E B Gueguim^*

来源：Industrial Crops and Products, 2017, 108: 219-224.

DOI：10.1016/j.indcrop.2017.06.048

• Pretreatment
• Salt
• Sugarcane leaves
• Lignocellulosic biomass

This study compares the optimization of combined and two-stage salt-acid pretreatment technique for efficient delignification, hemicellulose removal and enhancement of sugar yield from sugarcane leaf waste. The effect of process parameters ZnCl2 concentration (1 M-5 M), H2SO4 concentration (0.10-2.0%, v/v) and solid loading (5-15%, w/v) on the reducing sugar yield were investigated. The developed models showed coefficients of determination (R-2) > 0.94. The two-stage pretreatment under optimal conditions of 3.32 M ZnCl2, 1.84% (v/v) H2SO4, and 9.26% (w/v) solid loading gave 0.293 g/g reducing sugar, a 9% improvement compared to the optimized combined pretreatment. SEM and FTIR analysis showed major structural changes in the pretreated biomass. Significant lignin and hemicellulose removal was observed with the two-stage pretreatment. The optimized two-stage pretreatment also showed 66% and 194% yield improvement on sorghum leaves and Napier grass respectively. These results indicate that a two-stage ZnCl2-H2SO4 pretreatment can significantly enhance enzymatic saccharification of lignocellulosic waste.

• 出版日期2017-12-1