科研之友
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摘要
An on-line method was developed for the extraction and determination of fructans in plant samples. Fructans was extracted with water by ultrasound assisted, and directly introduced into a well of 96-well format which was reacted with concentrated sulphuric acid and 5% phenol for fast quantifying fructans. Response surface methodology (RSM) along with Box-Behnken design (BBD) was applied to optimize the critical variables of the extraction conditions of dynamic ultrasoundassisted extraction (DUAE) system for fructans yield from Kalimeris integrifolia. Three factors such as ultrasonic power, flow rate of solvent and volume of solvent were investigated. The optimum extraction conditions were ultrasonic power 496 W, flow rate of solvent 0. 63 mL/min, and volume of solvent 4. 69 mL. Under these conditions, the maximum fructans yield was 17. 60%. In addition, the single-factor-test was employed to optimize the major experimental parameters for the phenol-sulphuric acid procedure on 96-well microplate. The calibration curves of the proposed method under the optimised conditions corresponded very closely to a linear relationship (R >= 0. 99) over the concentration range from 20 to 100 mg/L for inulin. RSD of the method were 2. 9%-4. 1%, respectively. In this study, only small amount of sample, i. e. 5 mg, can meet the demand of the extraction. All of the analysis steps were completed in less than 15 min, and 96 samples could be analysed in 1 h using a 96-well format.
- 出版日期2011-3
- 单位东北师范大学
