Lymphatic Vessels and High Endothelial Venules are Increased in the Sentinel Lymph Nodes of Patients with Oral Squamous Cell Carcinoma Before the Arrival of Tumor Cells

作者：Chung Man Ki^*; Do In Gu; Jung Eunwook; Son Young Ik; Jeong Han Sin; Baek Chung Hwan

来源：Annals of Surgical Oncology, 2012, 19(5): 1595-1601.

DOI：10.1245/s10434-011-2154-9

To investigate the change of vasculature in the sentinel lymph node (SLN) of patients with oral squamous cell carcinoma. %26lt;br%26gt;Immunohistochemical staining of SLNs in 58 patients was performed with two monoclonal antibodies (MAb): anti-D2-40 MAb for lymphatic endothelial cells, and anti-MECA-79 MAb for high endothelial venules (HEV). Twelve metastatically involved (m(+)) SLNs, 120 uninvolved (m(-)) SLNs, and 35 non-SLNs (control) were available for analyses. Vessel densities were measured by computer-assisted analyses in the entire region of SLN. Correlations were assessed between vessel density and clinicopathologic variables, including vascular endothelial growth factor C of primary tumor. %26lt;br%26gt;Lymphatic vessel density (LVD) in SLNs was higher than that in control LNs [2361.8 mu m(2)/high-power field (HPF) (624.3-4758.5) vs. 1621.9 mu m(2)/HPF (465.3-3453.5), P = 0.005]. LVD of m(-) SLNs [2662.4 mu m(2)/HPF (624.3-4758.5)] and m(+/-) SLNs [4946.6 mu m(2)/HPF (2009.3-8698.8)] were both statistically significantly higher compared to control. HEV densities in m(-) SLNs [14029.7 mu m(2)/HPF (10465.7-17927.1)] as well as m(+/-) SLNs [18258.5 mu m(2)/HPF (8408.9-27706.0)] were also significantly higher than those in control [10350.5 mu m(2)/HPF (7807.8-12541.1)]. By multivariate analysis, the degree of vascular endothelial growth factor C expression of primary tumor showed significant correlation with LVD of SLNs (odds ratio 9.46, 95% confidence interval 1.73-51.5, P = 0.009), which was not the case in HEV. %26lt;br%26gt;Lymphatic vessels and HEVs were increased in SLNs, regardless of metastatic status of SLNs. Vascular endothelial growth factor C expression of primary tumor may contribute to the premetastatic change within SLNs of oral squamous cell carcinoma.

• 出版日期2012-5