Molecular cloning and expression of progestin membrane receptor component 1 (Pgrnrc1) of the giant tiger shrimp Penaeus monodon

作者：Preechaphol Rachanimuk; Klinbunga Sirawut^*; Yamano Keisuke; Menasveta Piamsak

来源：General and Comparative Endocrinology, 2010, 168(3): 440-449.

DOI：10.1016/j.ygcen.2010.06.002

• RACE-PCR
• Real-time PCR
• Ovarian development
• Shrimp
• Pgmrc1

Knowledge on molecular mechanisms of steroid hormonal induction on oocyte development may lead to the possible ways to effectively induce ovarian maturation in shrimp. In this study, progestin membrane receptor component 1 (Pgmrc1) of the giant tiger shrimp (Penaeus monodon) initially identified by EST analysis was further characterized. The full-length cDNA of Pgmrcl was 2015 bp in length containing an ORF of 573 bp corresponding to a polypeptide of 190 amino acids. Northern blot analysis revealed a single form of Pgmrc1 in ovaries of P. monodon. Quantitative real-time PCR indicated that the expression level of Pgmrc1 mRNA in ovaries of both intact and eyestalk-ablated broodstock was greater than that of juveniles (P < 0.05). Pgmrcl was up-regulated in mature (stage IV) ovaries of intact broodstock (P < 0.05). Unilateral eyestalk ablation resulted in an earlier up-regulation of Pgmrc1 since the vitellogenic (II) ovarian stage. Moreover, the expression level of Pgmrc1 in vitellogenic, early cortical rod and mature (II-1V) ovaries of eyestalk-ablated broodstock was greater than that of the same ovarian stages in intact broodstock (P< 0.05). Pgmrcl mRNA was clearly localized in the cytoplasm of follicular cells, previtellogenic and early vitellogenic oocytes. lmmunohistochemistry revealed the positive signals of the Pgmrc1 protein in the follicular layers and cell membrane of follicular cells and various stages of oocytes. Taken the information together, Pgmrcl gene products seem to play the important role on ovarian development and may be used as the bioindicator for monitoring progression of oocyte maturation of P. monodon.

• 出版日期2010-9-15