A novel alkaline keratinase from Bacillus subtilis DP1 with potential utility in cosmetic formulation

作者：Sanghvi Gaurav^*; Patel Harshit; Vaishnav Devendra; Oza Tejas; Dave Gaurav; Kunjadia Prashant; Sheth Navin

来源：International Journal of Biological Macromolecules, 2016, 87: 256-262.

DOI：10.1016/j.ijbiomac.2016.02.067

• Bacillus subtilis DP1
• Alkaline keratinase
• Dehairing cream
• CD spectra

The Bacillus subtilis DP1 was isolated from poultry farm soil at Anand district, India. The highest enzyme production (379.65 U/ml) was obtained at pH 10.0, a temperature of 37 degrees C and a growth period of 72 h. The extracellular keratinase was purified by gel filtration chromatography with 27.98 purification fold. Purity was also confirmed by High-Performance Liquid Chromatography (HPLC) analysis, where a major peak having retention time of 2.5 min was obtained on C18 column using photo diode array detector. Purified keratinase was stable in a broad range of pH (8-12) and temperature (20-50 degrees C) with optimum at pH 10.0 and 37 degrees C. The metallic ions, Ca2+ and Mg2+ enhance keratinase activity. Secondary structure from Circular Dichroism (CD) spectra implies that purified keratinase is largely beta-pleated sheet rich protein. For preparation of dehairing cream formulation, compatibility studies of excipients were carried out. Fourier transform infrared spectroscopy (FTIR) spectra of sodium stearate, calcium carbonate and sodium lauryl sulphate shows no reactivity of functional groups and hence mixture was compatible for formulation of keratinase dehairing cream. Prepared biological depilatory was able to remove hair more efficiently compared to marketed formulations.

• 出版日期2016-6