AN ALTERNATIVELY SPLICED FORM OF HLA-G MESSENGER-RNA IN HUMAN TROPHOBLASTS AND EVIDENCE FOR THE PRESENCE OF HLA-G TRANSCRIPT IN ADULT LYMPHOCYTES

作者：KIRSZENBAUM M^*; MOREAU P; GLUCKMAN E; DAUSSET J; CAROSELLA E

来源：Proceedings of the National Academy of Sciences of the United States of America, 1994, 91(10): 4209-4213.

DOI：10.1073/pnas.91.10.4209

• NONCLASSICAL CLASS I MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGEN
• FIRST TRIMESTER TROPHOBLASTS
• ADULT PERIPHERAL BLOOD

The HLA-G monomorphic, nonclassical class I gene encodes the major histocompatibility complex (MHC) molecule, which is the only MHC antigen expressed on cytotrophoblast cells of placenta. In this work, we have investigated expression of the HLA-G gene in fetal tissues and adult peripheral blood cells by using a sensitive hot-start reverse transcriptase PCR technique. PCR amplification with HLA-G primers specific for exon 3 has enabled us to demonstrate an alternatively spliced form of HLA-G mRNA present in fetal first trimester trophoblasts and lacking exon 4 (HLA-G.3-5). This low abundance transcript (approximate to 1:200) in comparison to full-length mRNA may encode the protein that excludes the alpha 3 domain and by conformational changes may present a different ability to bind to peptides. Moreover, expression of the HLA-G transcript was found in adult peripheral lymphocytes and equally in B- and T-cell populations. These results are discussed in the context of the fetal-maternal relationship presented by HLA-G gene products.

• 出版日期1994-5-10
• 单位中国地震局