Micro RNA Expression and Prognosis in Low-grade Non-invasive Urothelial Carcinoma

作者：Dip Nelson^*; Reis Sabrina T; Abe Daniel K; Viana Nayara I; Morais Denis R; Moura Caio M; Katz Betina; Silva Iran A; Srougi Miguel; Leite Katia R M

来源：International Brazilian Journal of Urology, 2014, 40(5): 644-649.

DOI：10.1590/S1677-5538.IBJU.2014.05.09

• MicroRNAs
• Urinary Bladder Neoplasms
• Prognosis
• Molecular Biology

Purpose: To analyze a possible correlation between a miRNA expression profile and important prognostic factors for pTa urothelial carcinomas (UC), including tumor size, multiplicity and episodes of recurrence.
Materials and Methods: Thirty low-grade non-invasive pTa bladder UC from patients submitted to transurethral resection were studied, in a mean follow-up of 17.7 months. As controls, we used normal bladder tissue from five patients submitted to retropubic prostatectomy to treat benign prostatic hyperplasia. Extraction, cDNA and amplification were performed for 14 miRNAs (miR-100, -10a, -21, -205, -let7c, -143, -145, -221, -223, -15a, -16, -199a and -452) using specific kits, and RNU-43 and -48 were used as endogenous controls. Statistical tests were used to compare tumor size, multiplicity and episodes of recurrence with miRNAs expression profiles.
Results: There was a marginal correlation between multiplicity and miR-let7c over- expression. For all others miRNA no correlation between their expression and prognostic factors was found.
Conclusion: We did not find differences for miRNAs expression profiles associated with prognostic factors in tumor group studied. The majority of miRNAs are down-regulated, except miR-10a, over-expressed in most of cases, seeming to have increased levels in tumor with more unfavorable prognostic factors. More studies are needed in order to find a miRNA profile able to provide prognosis in pTa UC to be used in clinical practice.

• 出版日期2014-10