Adaptation to tert-butyl hydroperoxide at a plasma membrane level in the fission yeast Schizosaccharomyces pombe parental strain and its t-BuOOH-resistant mutant

作者：Kalman Nikoletta; Gazdag Zoltan; Certik Milan; Belagyi Jozsef; Selim Samy A; Pocsi Istvan; Pesti Miklos^*

来源：Journal of Basic Microbiology, 2014, 54(3): 215-225.

DOI：10.1002/jobm.201200580

• Oxidative stress
• Plasma membrane
• Resistance
• Tert-butyl hydroperoxide
• Yeast

The one-gene mutant hyd1-190 of the fission yeast Schizosaccharomyces pombe displayed four-fold resistance to tert-butyl hydroperoxide (t-BuOOH) in comparison with its parental strain hyd(+). The cells of hyd1-190 exhibited a quantitative alteration in the sterol content and hence in the fatty acid composition of the plasma membrane, reflected in a two-fold amphotericin B sensitivity, increased rigidity of the plasma membrane, revealed by an elevated (7.9 degrees C) phase-transition temperature, measured by means of electron paramagnetic resonance spectroscopy, and a significantly decreased uptake of glycerol. Treatment of the strains with a subinhibitory concentration (0.2mM) of t-BuOOH induced adaptation via modification of the sterol and fatty acid compositions, resulting in increased (3.95 degrees C) and decreased (6.83 degrees C) phase-transition temperatures of the hyd(+) and hyd1-190 strains, respectively, in order to defend the cells against the consequences of t-BuOOH-induced external oxidative stress. However, in contrast with hyd(+), hyd1-190 lacks the ability to adapt to t-BuOOH at a cell level.

• 出版日期2014-3