New potential biomarkers for mesterolone misuse in human urine by liquid chromatography quadrupole time-of-flight mass spectrometry

作者：Lu Jianghai^*; Fernandez Alvarez Maria; Yang Sheng; He Genye; Xu Youxuan; Aguilera Rodigo

来源：Journal of Mass Spectrometry, 2015, 50(1): 153-159.

DOI：10.1002/jms.3508

• mesterolone
• new metabolites
• doping control
• human urine
• liquid chromatography quadruple time-of-flight mass spectrometry

In this paper, mesterolone metabolic profiles were investigated carefully. Mesterolone was administered to one healthy male volunteer. Urinary extracts were analyzed by liquid chromatography quadruple time-of-flight mass spectrometry (LC-QTOFMS) for the first time. Liquid-liquid extraction was applied to processing urine samples, and dilute-shoot analyses of intact metabolites were also presented. In LC-QTOFMS analysis, chromatographic peaks for potential metabolites were hunt down by using the theoretical [M-H](-) as target ions in full scan experiment, and their actual deprotonated ions were analyzed in targeted MS/MS mode. Ten metabolites including seven new sulfate and three glucuronide conjugates were found for mesterolone. Because of no useful fragment ion for structural elucidation, gas chromatography-mass spectrometry instrumentation was employed to obtain structural details of the trimethylsilylated phase I metabolite released after solvolysis. Thus, their potential structures were proposed particularly by a combined MS approach. All the metabolites were also evaluated in terms of how long they could be detected, and S1 (1-methyl-5-androst-3-one-17-sulfate) together with S2 (1-methyl-5-androst-17-one-3-sulfate) was detected up to 9days after oral administration, which could be the new potential biomarkers for mesterolone misuse.

• 出版日期2015-1
• 单位国家体育总局反兴奋剂中心