Anti-gal antibodies in a1,3-galactosyltransferase gene-knockout pigs

作者：Fang Jason; Walters Anneke; Hara Hidetaka; Long Cassandra; Yeh Peter; Ayares David; Cooper David K C^*; Bianchi John

来源：Xenotransplantation, 2012, 19(5): 305-310.

DOI：10.1111/j.1399-3089.2012.00710.x

• antibodies
• anti-galactose-a1
• 3-galactose
• galactose-a1
• 3-galactose
• pigs
• a1
• 3-galactosyltransferase gene-knockout

Fang J, Walters A, Hara H, Long C, Yeh P, Ayares D, Cooper DKC, Bianchi J. Anti-gal antibodies in a1,3-galactosyltransferase gene-knockout pigs. Xenotransplantation 2012; 19: 305310. (c) 2012 John Wiley & Sons A/S. Abstract Serum anti-galactose-a1,3-galactose (Gal) IgM and IgG antibody levels were measured by ELISA in a1,3-galactosyltransferase gene-knockout (GTKO) pigs (78 estimations in 47 pigs). A low level of anti-Gal IgM was present soon after birth, and rose to a peak at 46 m, which was maintained thereafter even in the oldest pigs tested (at >2 yr). Anti-Gal IgG was also present at birth, peaked at 3 m, and after 6 m steadily decreased until almost undetectable at 20 m. No differences in this pattern were seen between pigs of different gender. Total IgM followed a similar pattern as anti-Gal IgM, but total IgG did not decrease after 6m. The data provide useful baseline data for future experimental studies in GTKO pigs, e.g., relating to the antibody response to WT pig allografts.

• 出版日期2012-10