A novel multiplex PCR method for Clostridium botulinum neurotoxin type A gene cluster typing

作者：Umeda Kaoru; Seto Yoshiyuki; Kohda Tomoko; Mukamoto Masafumi; Kozaki Shunji^*

来源：Microbiology and Immunology, 2010, 54(5): 308-312.

DOI：10.1111/j.1348-0421.2010.00213.x

• boNT
• A gene cluster typing
• infant botulism
• multiplex PCR
• PFGE

A rapid, simple and sensitive multiplex PCR method for boNT/A gene cluster typing was developed by combining the results of BoNT/A subtype (boNT/A1 or /A2) gene detection with ha33 and/or p47 gene detection. Ten isolates associated with infant botulism in Japan were examined and divided into boNT/A gene cluster types 2 and 3 by origin (honey feeding or not) and period (1986-1987 or 1999-2007). It is suggested that this multiplex PCR method will be be useful for epidemiological studies of botulism.

• 出版日期2010-5