AN EPR SPIN-TRAPPING STUDY OF FREE RADICALS IN CIGARETTE SMOKE

作者：Ariciu Ana Maria; Ionita Gabriela; Ionita Petre^*

来源：Romanian Journal of Chemistry, 2014, 59(9): 781-787.

• cigarette smoke
• spin-trapping
• DMPO
• PBN

Tobacco smoking represents a source of exposure of humans to the reactive radicals which are considered responsible for chronic diseases. In this work we present the evidence of reactive radicals formation in tobacco smoke using the electron paramagnetic resonance (EPR) spin-trapping method. Four types of commercially available cigarette were used. In each case the cigarette was smoked a quarter, half and totally, and the smoke was passed through a filter into a toluene solutions of the spin traps alpha-phenyl-N-t-butylnitrone (PBN) and 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The analysis of the spin-adducts spectra evidenced the formation of different free radicals with short lives (C center dot, CO center dot, O center dot and HO center dot). The ratio between these radical species depends on the cigarette characteristics (tar and nicotine) and the cigarette length consumption. It was found that the cigarette tar gives also an EPR signal, corresponding to quinone type radicals.

• 出版日期2014-9