DEVELOPMENT OF MICROSATELLITES FROM FOTHERGILLA XINTERMEDIA (HAMAMELIDACEAE) AND CROSS TRANSFER TO FOUR OTHER GENERA WITHIN HAMAMELIDACEAE

作者：Hatmaker E Anne; Wadl Phillip A^*; Mantooth Kristie; Scheffler Brian E; Ownley Bonnie H; Trigiano Robert N

来源：Applications in Plant Sciences, 2015, 3(4): 1400123.

DOI：10.3732/apps.1400123

• Corylopsis
• Hamamelidaceae
• Hamamelis
• Loropetalum
• Parrotia
• simple sequence repeats

Premise of the study: We developed microsatellites from Fothergilla Xintermedia to establish loci capable of distinguishing species and cultivars, and to assess genetic diversity for use by ornamental breeders and to transfer within Hamamelidaceae.
Methods and Results: We sequenced a small insert genomic library enriched for microsatellites to develop 12 polymorphic microsatellite loci. The number of alleles detected ranged from four to 15 across five genera within Hamamelidaceae. Shannon's information index ranged from 0.07 to 0.14.
Conclusions: These microsatellite loci provide a set of markers to evaluate genetic diversity of natural and cultivated collections and assist ornamental plant breeders for genetic studies of five popular genera of woody ornamental plants.

• 出版日期2015-4