Mechanisms involved in the cytosolic calcium ion elevation induced by activating factors in chicken and rat phagocytes

作者：Yong Sheng; Abe Asaki; Kondo Yasuhiro^*

来源：Animal Science Journal, 2007, 78(1): 70-76.

DOI：10.1111/j.1740-0929.2006.00407.x

• chicken phagocyte
• cytosolic calcium ion
• formyl-methionyl-leucyl-phenylananine
• leukotriene B4
• phorbol myristate acetate

The aim of the present study was to determine the mechanism of cytosolic calcium ion concentration [Ca2+](i) elevation in chicken and rat phagocytes stimulated with phorbol myristate acetate (PMA), leukotriene B-4 (LTB4), formyl-methionyl-leucyl-phenylananine (fMLP) and Saccaromyces cerevisiae culture supernatant (SCS). Pretreatment with EGTA completely suppressed the PMA-induced [Ca2+](i) elevation in rat and chicken phagocytes, suggesting that all the [Ca2+](i) elevation induced in the PMA-stimulated rat and chicken phagocytes was attributable to the influx of extracellular Ca2+. On the other hand, the elevation of LTB4-, FMLP- and SCS-induced [Ca2+](i) was only partially suppressed by ethyleneglycol-bis (beta-aminoethyl)-N,N,N',N'-tetraacetic acid ethylene (EGTA) pretreatment of phagocytes. The results indicated that two pathways of [Ca2+](i) elevation, recruitment from the intracellular Ca2+ store and influx of extracellular Ca2+, are involved in the [Ca2+](i) elevation of LTB4-, fMLP- and SCS-stimulated phagocytes. In fMLP-stimulated rat neutrophils, [Ca2+](i) elevation showed a two-phase pattern in which the time lag between the first and second phase was approximately 1 min. The EGTA treatment of the fMLP-stimulated cells induced a reduction of the first phase level and a disappearance of the second phase. The reason for the special influence of EGTA observed in fMLP-stimulated cells is unknown, but the disappearance of the second phase of the [Ca2+](i) may be elicited by the EGTA-induced decrease of the first phase [Ca2+](i) elevation that depends on IP3 and diacylglycerol induced by fMLP.

• 出版日期2007-2
• 单位内蒙古工业大学