Adaption to stress via Pbs2 during Metarhizium rileyi conidia and microsclerotia development

作者：Wang, Zhongkang; Song, Zhangyong; Zhong, Qiang; Du, Fang; Yin, Youping^*

来源：World Journal of Microbiology and Biotechnology, 2018, 34(8): 107.

DOI：10.1007/s11274-018-2475-y

• Microsclerotia
• Metarhizium rileyi
• Morphology
• Conidiation
• Stress response

The high osmolarity glycerol (HOG) pathway plays important role in Metarhizium rileyi microsclerotia (MS) development. To investigate how M. rileyi transduce growth stress and regulate MS development via mitogen-activated protein kinase kinase (MAPKK) Pbs2, phenotypic characterization of the yeast Pbs2 homolog were performed. Expression of pbs2 peaked when MS formation occurred day 3 in liquid amended medium. Compared with wild-type and complemented strains, deletion mutant of pbs2 (Delta pbs2) delayed dimorphic switch and vegetative growth, displayed sensitivities to various stress, and significantly reduced conidial (98%) and MS (40%) yields. Furthermore, transcription analysis showed that other genes of HOG signaling pathway were down-regulated in Delta pbs2 mutants. Insect bioassays revealed that Delta pbs2 mutants had decreased virulence levels in topical (24%) and injection (53%) bioassays. This study confirmed that Pbs2 play important roles in colony morphology, conidiation, stresses response and MS development in M. rileyi.

• 出版日期2018-8
• 单位重庆大学