科研之友
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摘要
Plant-pathogenic Rhizoctonia spp. are soil-borne fungi with world-wide distribution. Most Rhizoctonia spp. are multinuclear and heterokaryotic fungi, which makes it difficult to build a stable transformation system. In this study, one uninucleate Rhizoctonia isolate, JN, was recovered from a field maize plant. Single nuclear status was observed under fluorescent microscopy after the hyphae were stained with Hoechst33258 solution. The optimal growth conditions of JN mycelia were 28 A degrees C at pH7.0 on potato dextrose agar (PDA) media. The mycelial color of JN cultured on PDA turned from white to light brown with aging. Analysis of the internal transcribed spacer (ITS) sequence of the ribosomal DNA (rDNA) showed that there were two types of ITS sequences within the single isolate and the nucleotide identity of the two sequences is only 95 %. JN is more sensitive to SDS, PEG4000, Sorbitol, hydrogen peroxide (H2O2) and sodium chloride (NaCl) than a multinucleate Rhizoctonia solani, XN5, cultured on PDA. Given the single nucleus, JN has great potential to be used in fungal transformation.
