A combination HIV reporter virus system for measuring post-entry event efficiency and viral outcome in primary CD4+T cell subsets

作者：Tilton Carisa A; Tabler Caroline O; Lucera Mark B; Marek Samantha L; Ha**ani Aiman A; Tilton John C^*

来源：Journal of Virological Methods, 2014, 195: 164-169.

DOI：10.1016/j.jviromet.2013.08.029

• HIV
• Reporter virus
• Fusion
• LTR
• Post-entry event efficiency
• Outcome

Fusion between the viral membrane of human immunodeficiency virus (HIV) and the host cell marks the end of the HIV entry process and the beginning of a series of post-entry events including uncoating, reverse transcription, integration, and viral gene expression. The efficiency of post-entry events can be modulated by cellular factors including viral restriction factors and can lead to several distinct outcomes: productive, latent, or abortive infection. Understanding host and viral proteins impacting post-entry event efficiency and viral outcome is critical for strategies to reduce HIV infectivity and to optimize transduction of HIV-based gene therapy vectors. Here, we report a combination reporter virus system measuring both membrane fusion and viral promoter-driven gene expression. This system enables precise determination of unstimulated primary CD4+ T cell subsets targeted by RIV, the efficiency of post-entry viral events, and viral outcome and is compatible with high-throughput screening and cell-sorting methods.

• 出版日期2014-1