A Comparative Analysis of Strict Kinetic Equations for the Enzyme Systems and Equations Obtained by the Rapid Equilibrium Approach

作者：Molina Alarcon M; Garcia Moreno M; Garcia Sevilla F; Masia M D; Amo Saus M Ll; Masia J; Ortiz Ruiz C V; Varon R^*

来源：MATCH-Communications in Mathematical and in Computer Chemistry, 2016, 75(3): 595-612.

The derivation of equations, which give the concentration of enzyme or ligand species in both the steady-state and the transient phase for a given mechanism of an enzyme reaction,is an unavoidable task for most kinetic analyses of any enzyme reaction. The above-mentioned equations can be obtained either under strict conditions, i.e. without the assumption of reversible steps being in rapid equilibrium, or by assuming a rapid equilibrium of one of thereversible step, or more,involved in the reaction mechanism (partial rapid equilibrium approach), or them all(total rapid equilibrium approach). Both equation types, strict ones and those obtained by assuming arapid equilibrium, which formally differ for the same reaction mechanismin many cases, have advantages and limitations, which are discussed herein with specific examples.

• 出版日期2016