A comparative study of transfection of rat mesenchymal stem cells using polyethyleneimine-coated magnetic ferro-ferric oxide nanoparticles and lipofectamine

作者：Liu, Chao; Yu, Yijun; Miao, Leiying^*; Liu, Yu; Sun, Weibin^*

来源：International Journal of Clinical and Experimental Medicine, 2016, 9(3): 6062-6069.

• Mesenchymal stem cells
• stem cells
• transfection
• magnetic nanoparticles
• lipofectamine
• gene therapy

Stem cell transfection agents have traditionally included lipofectamine, but newer agents include polyethyleneimine-coated magnetic ferro-ferric oxide nanoparticles. This study was done to compare these two methods in transfecting cultured ratmesenchymal stem cells. Magnetic ferro-ferric oxide nanoparticles were coated with polyethyleneimine using co-precipitation of alkali metal ions, forming a mobility diameter of approximately 50 nm. It dispersed well in water. Compared to the lipofectamine, polyethyleneimine-coated magnetic ferro-ferric oxide nanoparticles had higher transfection rate-in rat mesenchymal stem cells (P<0.05). Meanwhile, cell proliferation analysis revealed less toxicity of such nanoparticles to rat mesenchymal stem cells. In summary, polyethyleneimine-coated magnetic ferro-ferric oxide nanoparticles are safe and had a higher transfection rate in cultured ratmesenchymal stem cells, indicating that this improved technique of mesenchymal stem cell transfection may have clinical application potential.

• 出版日期2016
• 单位南京大学