Molecular and phenotypic characterization of Xenorhabdus bovienii symbiotically associated with Steinernema silvaticum

作者：Kazimierczak Waldemar^*; Sajnaga Ewa; Skowronek Marcin; Kreft Anna Maria; Skrzypek Henryk Wojciech; Wiater Adrian

来源：Archives of Microbiology, 2016, 198(10): 995-1003.

DOI：10.1007/s00203-016-1261-1

• Xenorhabdus bovienii
• Steinernema silvaticum
• Entomopathogenic nematodes
• Symbiosis
• Phylogeny

Steinernema silvaticum is a common entomopathogenic nematode in soil of Europe; however, little is known about the bacteria living in symbiosis with this animal. In this study, we have isolated four bacterial strains from S. silvaticum and identified them as members of the species Xenorhabdus bovienii. This study was based on 16S rRNA and concatenated recA, dnaN, gltX, and gyrB gene sequence analysis. In addition, phenotypic traits have been considered, indicating that the tested strains are the most similar to those of X. bovienii. The phylogenetic relationships between the isolated strains and other strains of X. bovienii derived from various nematode hosts were analyzed and discussed. This is the first report confirming the symbiotic association of X. bovienii with S. silvaticum.

• 出版日期2016-12