Anaerobic testosterone degradation in Steroidobacter denitrificans - Identification of transformation products

作者：Fahrbach Michael; Krauss Martin; Preiss Alfred; Kohler Hans Peter E; Hollendera Juliane^*

来源：Environmental Pollution, 2010, 158(8): 2572-2581.

DOI：10.1016/j.envpol.2010.05.017

• Testosterone
• Steroid
• Biodegradation
• Denitrification
• Transformation product

The transformation of the androgenic steroid testosterone by gammaproteobacterium Steroidobacter denitrificans was studied under denitrifying conditions. For the first time, growth experiments showed that testosterone was mineralized under consumption of nitrate and concurrent biomass production. Experiments with cell suspensions using [4-(14)C]-testosterone revealed the intermediate production of several transformation products (TPs). Characterisation of ten TPs was carried out by means of HPLC coupled to high resolution mass spectrometry with atmospheric pressure chemical ionization as well as (1)H and (13)C NMR spectroscopy. 3 beta-hydroxy-5 alpha-androstan-17-one (trans-androsterone) was formed in the highest amount followed by 5 alpha-androstan-3,17-dione. The data suggests that several dehydrogenation and hydrogenation processes take place concurrently in ring A and D because no consistent time-resolved pattern of TP peaks was observed and assays using 2 TPs as substrates resulted in essentially the same TPs. The further transformation of testosterone in S. denitrificans seems to be very efficient and fast without formation of detectable intermediates.

• 出版日期2010-8