Modulation of adipogenesis-related gene expression by ethanol extracts of Detam 1 soybean and Jati belanda leaf in 3T3-L1 cells

作者：Hidayat Meilinah; Prahastuti Sijani; Fauziah Nurul; Maesaroh Maesaroh; Balqis Balqis; Widowati Wahyu

来源：Bangladesh Journal of Pharmacology, 2016, 11(3): 697-702.

DOI：10.3329/bjp.v11i3.26471

<jats:p>&lt;p class="Abstract"&gt;In this study, we evaluated the effects of ethanol extracts of Detam 1 soybean, Jati belanda leaf, and the combination toward expression of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), and stearoyl-CoA desaturase 1 (SCD1) genes in 3T3-L1 cells as anti-adipogenesis and anti-obesity. The differentiation of 3T3-L1 cells into adipocyte was conducted using induction medium consist of Dulbecco's Modified Eagle's Medium, 3-isobutyl-1-methylxanthine, insulin, dexamethasone, and fetal bovine serum. The expression of PPARγ, C/EBPα, and SCD1 gene was measured using real-time quantitative polymerase chain reaction (qPCR). Ethanol extract of Jati belanda at a concentration of 50 μg/mL was most effective to reduce PPARγ, C/EBPα, and SCD1 gene expression in 3T3-L1 cells. Ethanol extract of Detam 1 soybean failed to reduce PPARγ gene expression, whilst in the concentration of 50 μg/mL it was able to significantly reduce the C/EBPα and SCD1 gene expression. Both ethanol extracts of Detam 1 soybean and Jati belanda have potential as anti-adipogenesis and anti-obesity by suppressing adipogenesis-related gene expression, particularly C/EBPα and SCD1.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Video Clip:&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;&lt;a href="https://youtube.com/v/UWv9nChmF2Y"&gt;Maintaining 3T3-L1 cell culture&lt;/a&gt;: 5 min 15 sec&lt;/p&gt;</jats:p>

• 出版日期2016