Application of Immunomagnetic Separation to E. coli O157: H7 Detection

作者：Tang Qian-qian; Wang Jian-ping^*; Ye Zun-zhong; Gai Ling; Ying Yi-bin

来源：Spectroscopy and Spectral Analysis, 2009, 29(10): 2614-2618.

DOI：10.3964/j.issn.1000-0593(2009)10-2614-05

• E. coli O157: H7
• Immunomagnetic separation
• ATP bioluminescence
• Capture efficiency
• Colony counting

In the present paper, the capture efficiencies of four different proportions of E. coli O157 : H7 and immunomagnetic beads (IMB) (1: 6. 34, 1 : 12. 69, 1 : 25. 38 and 1 : 30. 07) and three different proportions of E. coli O157 : H7 and Bacillus subtilis (0. 3 mL : 0. 1 mL, 0. 2 mL : 0. 2 mL and 0. 1 mL : 0. 3 mL) were compared, with the total volume and capacity of IMB remaining constant. It was revealed that the capture efficiency could reach 100% when the proportion of E. coli O157 : H7 and IMB was 1 : 30, meaning that almost all targets E. coli O157 : H7 could be captured at 1 : 30, and that the capture efficiency decreased and then increased with the number of Bacillus subtilis increasing. Moreover, the combination of ATP bioluminescence and IMS was correlated well with conventional culture methods, R-2 = 0. 988 2, and the detection limit was as low as 10(2) CFU . mL(-1).

• 出版日期2009-10
• 单位浙江大学