Activation of matrix metalloproteinase-26 by HOXA10 promotes embryo Cross Mark adhesion in vitro

作者：Jiang Yue; Yan Guijun; Zhang Hui; Shan Huizhi; Kong Chengcai; Yan Qiang; Xue Bai; Diao Zhenyu; Hu Yali^*; Sun Haixiang

来源：Biochemical and Biophysical Research Communications, 2014, 445(3): 622-628.

DOI：10.1016/j.bbrc.2014.02.057

• MMP-26
• HOXA10
• Embryo adhesion

Successful embryonic implantation requires an effective maternal-embryonic molecular dialogue. However, the detailed mechanisms of epithelial-embryo adhesion remain poorly understood. Here, we report that matrix metalloproteinase-26 (MMP-26) is a novel downstream target gene of homeobox a 10 (HOXA10) in human endometrial cells. HOXA10 binds directly to a conserved TTAT unit (-442 to 439) located within the 5' regulatory region of the MMP-26 gene and regulates the expression and secretion of MMP-26 in a concentration-dependent manner. Moreover, the adenovirus-mediated overexpression of MMP-26 in Ishikawa cells markedly increased BeWo spheroid adhesion. An antibody blocking assay further demonstrated that the promotion of BeWo spheroid adhesion by HOXA10 and MMP-26 was significantly inhibited by pre-treatment with a specific antibody against MMP-26. These results demonstrate that the HOXA10-mediated expression of MMP-26 promotes embryo adhesion during the process of embryonic implantation.

• 出版日期2014-3-14
• 单位南京医科大学