Differential gene expression in peripheral blood T cells from patients with psoriasis, lichen planus, and atopic dermatitis

作者：Li, Xinhua; Li, Junqin; Yang, Yuanwen; Hou, Ruixia; Liu, Ruifeng; Zhao, Xincheng; Yan, Xin; Yin, Guohua; An, Peng; Wang, Yong; Zhang, Kaiming^*

来源：Journal of the American Academy of Dermatology, 2013, 69(5): E235-E243.

DOI：10.1016/j.jaad.2013.06.030

• atopic dermatitis
• differentially expressed genes
• lichen planus
• psoriasis
• regulation of nuclear remessenger RNA domain containing 1B
• T cells

Background: Psoriasis, lichen planus (LP), and atopic dermatitis (AD) are common chronic inflammatory skin diseases mediated by immune responses.
Objective: We used RNA sequencing to investigate messenger RNA expression patterns in peripheral T cells of Chinese patients with psoriasis, LP, or AD and of healthy individuals.
Methods: After peripheral T-cell proliferation, messenger RNA expression patterns were investigated by RNA sequencing, and 6 randomly selected genes were verified by real-time reverse transcriptase polymerase chain reaction.
Results: Six genes were down-regulated and 33 were up-regulated in these diseases. Gene ontology analysis revealed enrichment of genes involved in positive regulation of T-cell activation. Regulation of nuclear premessenger RNA domain containing 1B (RPRD1B) expression was enhanced in psoriasis.
Limitations: The role of hereditary factors in RPRD1B expression in T cells was not considered. Immunomodulators (thymopeptide, levamisole, BCG polysaccharide, nucleic acid injection, and transfer factor) were previously given to patients with psoriasis and LP, but not to patients with AD; the effects of these immunomodulators on gene expression is uncertain.
Conclusion: RPRD1B may be involved in T-cell activation in our Chinese psoriatic cohort, and may play a role in stimulating epidermal hyperproliferation.

• 出版日期2013-11
• 单位青海大学