The efficient synthesis and purification of amyloid-beta(1-42) using an oligoethylene glycol-containing photocleavable lysine tag

作者：Karas John A^*; Noor Asif; Schieber Christine; Connell Timothy U; Separovic Frances; Donnelly Paul S^*

来源：Chemical communications, 2017, 53(51): 6903-6905.

DOI：10.1039/c7cc03147e

A short, monodisperse oligoethylene glycol-containing photocleavable lysine tag was developed to facilitate the efficient purification of hydrophobic and fibril-forming peptides. This new tag was used to prepare a modified A beta(42) peptide with increased solubility and decreased propensity to aggregate in aqueous media. The solubilising tag was readily removed by irradiation with UV light and permitted the preparation and isolation of A beta(42) in high purity and yield.

• 出版日期2017-6-28