Wenxin Keli attenuates ischemia-induced ventricular arrhythmias in rats: Involvement of L-type calcium and transient outward potassium currents

作者：Wang, Xi; Wang, Xin; Gu, Yongwei; Wang, Teng; Huang, Congxin^*

来源：Molecular Medicine Reports, 2013, 7(2): 519-524.

DOI：10.3892/mmr.2012.1195

• ventricular arrhythmias
• L-type calcium current
• transient outward potassium current
• patch clamp techniques
• traditional Chinese medicines

Wenxin Keli is the first state-sanctioned traditional Chinese medicine (TCM)-based antiarrhythmic drug. The present study aimed to examine whether long-term treatment with Wenxin Keli reduces ischemia-induced ventricular arrhythmias in rats in vivo, and if so, which mechanisms are involved. Male rats were treated with either saline (control group) or Wenxin Keli for 3 weeks and were subjected to myocardial ischemia for 30 min with assessment of the resulting ventricular arrhythmias. The L-type calcium current (I-ca,I-L) and transient outward potassium current (I-to) were measured by the patch clamp technique in normal rat cardiac ventricular myocytes. During the 30-min ischemia, Wenxin Keli significantly reduced the incidence of ventricular fibrillation (VF) (P<0.05). The number of ventricular tachycardia (VT)+VF episodes and the severity of arrhythmias were significantly reduced by Wenxin Keli administration compared to the control group (P<0.05). In addition, Wenxin Keli inhibited I-Ca,I-L and I-to in a concentration-dependent manner. These results suggest that long-term treatment with Wenxin Keli may attenuate ischemia-induced Ventricular arrhythmias in rats and that I-Ca,I-L and I-to may be involved in this attenuation.

• 出版日期2013-2
• 单位武汉大学