Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents

作者：Carol Visser Jeroen; van der Schans Marcel; Fidder Alex; Huist Albert G; van Baar Ben L M; Irth Hubertus; Noort Daan^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2008, 870(1): 91-97.

DOI：10.1016/j.jchromb.2008.06.008

• chemical warfare agents
• protein adduct
• mass spectrometry

Rapid monitoring and retrospective verification are key issues in protection against and non-proliferation of chemical warfare agents (CWA). Such monitoring and verification are adequately accomplished by the analysis of persistent protein adducts of these agents. Liquid chromatography-mass spectrometry (LC-MS) is the tool of choice in the analysis of such protein adducts, but the overall experimental procedure is quite elaborate. Therefore, an automated on-line pepsin digestion-LC-MS configuration has been developed for the rapid determination of CWA protein adducts. The utility of this configu

• 出版日期2008-7-1