Molecular cloning, expression pattern and phylogenetic analysis of myosin light chain 2 gene from Antheraea pernyi: A potential marker for phylogenetic inference

作者：Liu, Lin; Wang, Hui-Ying; Jin, Hong-Yu; Wu, Song; Li, Yu-Ping; Liu, Yan-Qun^*; Li, Xi-Sheng; Qin, Li; Wang, Zhen-Dong

来源：Biochemical Systematics and Ecology, 2010, 38(5): 981-987.

DOI：10.1016/j.bse.2010.09.006

• Antheraea pernyi
• Myosin light chain 2
• Gene cloning
• Expression pattern
• Phylogeny

Myosin light chain 2 (MLC-2) gene was isolated and characterized from Antheraea pernyi, a well-known wild silkmoth. The isolated cDNA sequence is 905 bp in length with an open reading frame of 612 bp encoding a polypeptide of 203 amino acids. Semi-quantitative RT-PCR analysis showed that the MLC-2 gene was transcribed during four developmental stages (egg, larva, pupa, and moth), and present in all tissues tested. Alignment analysis revealed that the deduced protein sequence has over 95% identity to myosin light chain 2 of lepidopteran species, and 57-88% identity to other insect species, suggesting that insect MLC-2 proteins are highly conserved throughout evolution. The protein sequence was used to construct phylogenetic trees with other known vertebrate and invertebrate MLC-2 sequences, and the obtained trees demonstrated similar topology with the classical systematics, indicating the potential value of MLC-2 gene in phylogenetic, study.

• 出版日期2010-10
• 单位沈阳农业大学