科研之友
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摘要
Microorganisms from denim-manufacturing mill sludge (DMMS) were screened for their capacity to biodegrade indigo wastewater pretreated with laccase. A bacterial strain that utilizes isatin and anthranilic acid, the metabolites of indigo produced oxidatively by laccase, was isolated and characterized. Based on the 16S rRNA gene sequence and morphological, physiological and biochemical properties of the strain, it was identified as Achromobacter xylosoxidans N4. Anthranilic acid was detected by HPLC as a metabolite when isatin was added to the bacterial culture as the sole carbon and energy source. The N4 strain exhibited strong degradation of isatin and its metabolite anthranilic acid and was able to grow in MSM supplemented with 100 mg/L anthranilic acid with an initial pH value ranging from 4.0 to 9.0. This is the first report of a bacterium isolated from DMMS that can degrade isatin and anthranilic acid. These results indicate that the N4 strain has potential applications in the treatment of indigo wastewater.
