Development of a new set of molecular markers for examining Glu-A1 variants in common wheat and ancestral species

作者：Dong, Zhenying^*; Yang, Yushuang; Zhang, Kunpu; Li, Yiwen; Wang, Junjun; Wang, Zhaojun; Liu, Xin; Qin, Huanju; Wang, Daowen^*

来源：PLos One, 2017, 12(7): e0180766.

DOI：10.1371/journal.pone.0180766

• WEIGHT GLUTENIN SUBUNITS
• BREAD-MAKING QUALITY
• GENETIC DIVERSITY
• STORAGE PROTEINS
• GENOME EVOLUTION
• SEQUENCE
• LINES
• INSERTION
• ALLELES
• REGIONS

In common wheat (Triticum aestivum L.), allelic variations of Glu-A1 locus have important influences on grain end-use quality. Among the three Glu-A1 alleles, Glu-A1a and -A1b encode the high-molecular-weight glutenin subunits (HMW-GSs) 1Ax1 and 1Ax2*, respectively, whereas Glu-A1c does not specify any subunit. Here, we detected a total of 11 GluA1 locus haplotypes (H1 to H11) in three wheat species, by developing and using a new set of DNA markers (Xrj5, Xid3, Xrj6, Xid4 and Xrj7). The main haplotypes found in the diploid wheat T. urartu were H1, H4, H5 and H6, with H1 and H4 expressing both 1Ax and 1Ay subunits. The major haplotypes revealed for tetraploid wheat (T. turgidum) were H1, H8 and H9, with the lines expressing both 1Ax and 1Ay belonging to H1, H4 or H7. Four major haplotypes (H1, H9, H10 and H11) were discovered in common wheat, with Glu-A1a associated with H1 and H8, Glu-A1b with H10 or H11, and Glu-A1c with H9. The Glu-A1 locus haplotypes and the new set of DNA markers have potential to be used for more effectively studying and utilizing the molecular variations of Glu-A1 to improve the end-use quality of common wheat are discussed.

• 出版日期2017-7-6
• 单位中国科学院大学; 中国热带农业科学院; 河南农业大学; 中国热带农业科学院椰子研究所; 中国科学院遗传与发育生物学研究所