Aberrant methylation in promoter-associated CpG islands of multiple genes in therapy-related leukemia

作者：Uehara E; Takeuchi S; Tasaka T^*; Matsuhashi Y; Yang Y; Fujita M; Tamura T^*; Nagai M; Koeffler HP

来源：International Journal of Oncology, 2003, 23(3): 693-696.

• therapy-related leukemia
• AML
• promotor hypermethylation
• cell cycle control gene
• DNA repair gene

Methylation profile was analyzed in eleven cases of therapy-related leukemia (t-leukemia) for p14, p15, p16, Rb, hMLH1, hMSH2, MGMT, APC, RAR beta, DAPK, RIZ1, FHIT, and SOCS-1 genes by using methylation specific polymerase chain reaction (MSP) analysis. Six (55%) of eleven cases showed methylation of at least one gene. The average time to the development of t-leukemia after the treatment of the primary tumor was significantly shorter in patients with methylation than those without methylation (49.3 months vs. 133.2 months, P=0.044). These results suggest that hypermethylation might be involved in the development of t-leukemia.

• 出版日期2003-9