Analysis of prolonged storage on coagulation Factor (F)V, FVII, and FVIII in thawed plasma: is it time to extend the expiration date beyond 5 days?

作者：Tholpady Ashok^*; Monson Janet; Radovancevic Rajko; Klein Kimberly; Bracey Arthur

来源：Transfusion, 2013, 53(3): 645-650.

DOI：10.1111/j.1537-2995.2012.03786.x

BACKGROUND: According to AABB standards, fresh-frozen plasma (FFP) should be thawed at 30 to 37 degrees C and expire after 24 hours. An increase in the aggressive management of trauma patients with thawed plasma has heightened the risk of plasma waste. One way to reduce plasma waste is to extend its shelf life, given that the full range of therapeutic efficacy is maintained. We evaluated the effect of prolonged storage at 1 to 6 degrees C on the activity of Factor (F)V, FVII, and FVIII in plasma thawed at 37 or 45 degrees C. STUDY DESIGN AND METHODS: Group O plasma from healthy donors (n=20) was divided into 10 pairs and frozen and stored at not more than 18 degrees C. One sample from each pair was thawed at 37 or 45 degrees C, and all were stored at 1 to 6 degrees C. Samples were analyzed for FV, FVII, and FVIII activity on Days 0, 5, 10, 15, and 20. RESULTS: Plasma thawing time was 17% less at 45 degrees C than at 37 degrees C. No differences were observed between thawing groups in coagulation activity of FV, FVII, and FVIII during the 20-day storage period (p%26gt;0.12). In both groups, the activity of FV and FVIII decreased over time but remained within a normal range at 10 days. CONCLUSION: Although levels of plasma clotting factors are reduced in storage, therapeutic levels of FV and FVIII are maintained in thawed plasma stored for up to 10 days at 1 to 6 degrees C. Thawing of FFP at 45 degrees C decreases thawing time but does not affect the activity of FV, FVII, and FVIII.

• 出版日期2013-3