An automated multiwell plate reading film microscope for live cell autofluorescence lifetime assays

作者：Kelly Douglas J^*; Warren Sean C; Kumar Sunil; Lagarto Joao L; Dyer Benjamin T; Margineanu Anca; Lam Eric W F; Dun**y Chris; French Paul M W

来源：Journal of Innovative Optical Health Sciences, 2014, 7(5): 1450025.

DOI：10.1142/S1793545814500254

• High content analysis
• FLIM
• NADH
• metabolism
• cisplatin

Fluorescence lifetime imaging (FLIM) is increasingly used to read out cellular autofluorescence originating from the coenzyme NADH in the context of investigating cell metabolic state. We present here an automated multiwell plate reading FLIM microscope optimized for UV illumination with the goal of extending high content fluorescence lifetime assays to readouts of metabolism. We demonstrate its application to automated cellular autofluorescence lifetime imaging and discuss the key practical issues associated with its implementation. In particular, we illustrate its capability to read out the NADH-lifetime response of cells to metabolic modulators, thereby illustrating the potential of the instrument for cytotoxicity studies, assays for drug discovery and stratified medicine.

• 出版日期2014-9