A comparison of N-glycan profiles in human plasma and vitreous fluid

作者：Inafuku Saori; Noda Kousuke^*; Amano Maho; Ohashi Tetsu; Yoshizawa Chikako; Saito Wataru; Kanda Atsuhiro; Nishimura Shin Ichiro; Ishida Susumu

来源：Graefes Archive for Clinical and Experimental Ophthalmology, 2014, 252(8): 1235-1243.

DOI：10.1007/s00417-014-2671-x

• Glycan
• N-glycan
• Vitreous fluid
• Plasma

To investigate the concentration and composition of N-glycans in plasma and vitreous samples obtained from patients with non-proliferative vitreoretinal diseases. %26lt;br%26gt;Plasma and vitreous samples were collected from 11 patients with idiopathic macular hole (MH) and 9 patients with epiretinal membrane (ERM). The samples were pretreated for enzymatic cleaving, and subsequently glycans released from proteins were captured on BlotGlyco H beads. Sialic acids were methyl-esterified. Processed glycans were tagged with aminooxy-functionalized peptide reagent (aoWR) and released from the beads, followed by detection by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The concentration and composition of N-glycans in the samples were assessed. %26lt;br%26gt;Concentration of N-glycans in vitreous samples (132 +/- 29 pmol/100 mu g protein) was significantly lower compared with those in plasma samples (714 +/- 29 pmol/100 mu g protein, p %26lt; 0.001). Predominant N-glycan in both plasma (39.7 +/- 1.1 %) and vitreous fluid (37.2 +/- 3.1 %) was identical, and the composition was presumed as [(Hex)2(HexNAc)2(NeuAc)2+ (Man)3(GlcNAc)2]. By contrast, the second-ranked N-glycan in vitreous samples (15.6 +/- 1.5 %) was the seventh in plasma (2.3 +/- 0.2 %). %26lt;br%26gt;The current data provide useful information on N-glycan profile in the vitreous fluid, which is distinct from that in the plasma.

• 出版日期2014-8