Methylenetetrahydrofolate Reductase C677T and A1298C Polymorphism in Iranian Women With Idiopathic Recurrent Pregnancy Losses

作者：Yousefian Elham^*; Kardi Mohammad Taghi; Allahveisi Azra

来源：Iranian Red Crescent Medical Journal, 2014, 16(7): e16763.

DOI：10.5812/ircmj.16763

• Methylenetetrahydrofolate Reductase
• Thrombophilia
• Pregnancy

Background: Recurrent pregnancy loss (RPL) is a serious problem for pregnancy. There is evidence that vascular complications play a principal role in RPL. Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in folate metabolism. Polymorphisms (C677T,A1298C) of MTHFR gene are associated with decreased MTHFR activity.
Objectives: The aim of this study was to determine the association between MTHFR polymorphisms (C677T and A1298C) and recurrent pregnancy loss (RPL) in Iranian women.
Materials and Methods: In this case-control study, blood samples were obtained from patients who had three or more consecutive pregnancy losses before the 22nd week of pregnancy (n = 204). The control group consisted of 116 age-matched women with at least one alive child and without any history of pregnancy loss or other gestational complications (n =116). Following DNA extraction, samples were tested for MTHFRC677T and A1298C polymorphisms using the reverse hybridization method.
Results: The prevalence of 677TT mutation was 8.8% (18/204) in the patient group and 8.6% (10/116) in the control group (P = 0.434). The prevalence of 1298CC mutation was 123 % (25/204) in the patient group and 8% (9/116) in the control group (P = 0.155). Investigation of the distributions of various genotypes of MTHFRC677T and A1298C did not indicate a significant difference between patients with RPL and healthy control subjects.
Conclusions: The results suggest that WITHER mutations might not be associated with RPL in the examined population.

• 出版日期2014-7