A rhodamine-based fast and selective fluorescent probe for monitoring exogenous and endogenous nitric oxide in live cells

作者：Wang, Qing; Jiao, Xiaojie; Liu, Chang; He, Song; Zhao, Liancheng; Zeng, Xianshun^*

来源：Journal of Materials Chemistry B, 2018, 6(24): 4096-4103.

DOI：10.1039/c8tb00646f

• LIVING CELLS
• AQUEOUS-SOLUTION
• DEHYDROASCORBIC ACID
• RATIONAL DESIGN
• BIOLOGICAL-SYSTEMS
• IN-VITRO
• NO
• INDICATORS
• MITOCHONDRIA
• CHROMOPHORE

Nitric oxide, an important cellular messenger molecule, plays a wide range of physiological roles in neurotransmission, vascular relaxation, platelet activation, and immune response. In this work, we synthesized a selective and sensitive cationic fluorescent probe (ROPD) for monitoring exogenous and endogenous nitric oxide in live cells by grafting a NO-trapper o-phenylenediamine (OPD), via one of the amino groups of OPD, onto a rhodamine fluorophore at the C-3 position. The probe exhibited high selectivity and sensitivity towards NO with fast response in the water phase over a wide pH range. At the same time, the probe ROPD is cell-membrane permeable and can be used for the real-time visualization of exogenous NO in both L929 and HeLa cells, and endogenous NO in stressed RAW 264.7 cells.

• 出版日期2018-6-28
• 单位天津理工大学; 哈尔滨工业大学