科研之友
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摘要
Hederagenin (HD), a derivative of oleanolic acid extracted from the leaves of ivy (Hedera helix L.), exhibited a variety of biological activities, including potent antitumor properties both in vitro and in vivo. In our study, effects of the antitumor and apoptosis of HD on H-22 hepatoma bearing mice were conducted by immunofluorescence, RT-PCR and Western blot. The results showed that the tumor weights were reduced in treated groups compared with that of control group. The fluorescence intensity of the expression of Fas gene was increased in treated groups compared with that of the control group. Fluorescence intensity of the expression of Fasl, Caspase-3 and Caspase-8 gene were increased by HD and cyclophosphamide (CTX) treatments. The expression of Fas, FasL, Caspase-3 and Caspase-8 mRNA were increased compared with that of the control group. Protein expression of Fasl was increased markedly, whereas protein expression of Fas was rarely changed in the HD and CTX groups. Protein expression of Caspase-3 and Caspase-8 were increased in the HD groups. All the results showed that HD would play an important role in anti-tumor.
