Absence of lactobacilli containing glycolipids with the alpha-galactose epitope and the enhanced fucosylation of a receptor glycolipid GA1 in the digestive tracts of immune-deficient scid mice

作者：Iwamori Masao^*; Tanaka Kyoko; Adachi Shigeki; Aoki Daisuke; Nomura Taisei

来源：Journal of Biochemistry, 2015, 158(1): 73-82.

DOI：10.1093/jb/mvv021

• blood group
• digestive tract
• fucosyltransferase
• glycolipids
• lactobacillus

The Lactobacillus species in the digestive tracts of immune-deficient scid mice was distinct from that in control mice, i.e. Lactobacillus marinas in scid and L. johnsonii in control mice, according to their 16S-rRNA, indicating that a symbiotic relationship between lactobacilli and a host is established under pressure from the immune system. The caecal and colonal contents rich in L. marinas of scid mice were loose with a strong sour smell, resulting in diarrhoea, and those with L. johnsonii in control mice included abundant solid materials. Lactobacillus glycolipids were revealed to be recognized by the immune system, and by TLC-immunostaining, LacTetH-DG (Gal alpha 1-6Gal alpha 1-6Gal alpha 1-2Glc alpha 1-3'DG) of L. johnsonii was detected in the stomach, caecum and colon of control mice, but not in those of scid ones, in which fucosylation of a receptor GA1 for L. johnsonii was enhanced more than 4-fold compared with in the control mice. Thus, structural modification of receptor glycolipids was revealed to occur in the process of establishment of a symbiotic relationship between lactobacilli and a host. LacTetH-DG was also immunogenic to human, because of the presence of natural antibodies against it, and the antibody binding to it was comparable to that of blood group- and species-related glycosphingolipids.

• 出版日期2015-7