A coumarin-based fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 for the detection of cervical cancer

作者：He, Guangjie^*; Yang, Lu; Qian, Xinlai; Li, Jing; Yuan, Zhiqing^*; Li, Changzheng

来源：International Journal of Molecular Medicine, 2017, 39(6): 1571-1579.

DOI：10.3892/ijmm.2017.2974

• cervical cancer
• matrix metalloproteinase-2
• fluorescence resonance energy transfer
• fluorescent probe
• coumarin

Cervical cancer is one of the most common gynecological malignancies worldwide. At present, the methods used for cervical cancer screening have many disadvantages. Matrix metalloproteinase-2 (MMP-2) is low or absent in normal cervical epithelial cells while overexpressed in cervical intraepithelial neoplasia and cervical cancer, which provides the possibility for the detection of cervical cancer based on MMP-2. The development of a strategy with high sensitivity and specificity for cervical cancer detection would be expected to improve the cure rate. Connecting a fluorophore [7-(diethylamino)-2-oxo-2H-chromene-3-succinimidyl ester] with a quencher 14[4-(dimethylamino)phenylazol benzoic acid N-succinimidyl ester} via an MMP-2 substrate peptide GPLGVRGKGG, a new coumarin-based fluorescence resonance energy transfer probe targeting MMP-2 was prepared to examine cervical cancer by cell imaging. The cervical cancer cell lines showed green fluorescence with intensities in the order of CaSki > SiHa > C33A > HeLa, which indicated that the cervical cancer cell lines possessed significantly differential expression levels of MMP-2. Quantitative (real-time) PCR, RT-PCR, and western blot experiments were consistent with such results. The fluorescence detection limit of the probe for MMP-2 was estimated to be 0.05 ng/ml. Therefore, this MMP-2 probe potentially provides a sensitive and specific visual method for cervical cancer screening, diagnosis and prognostic judgment.

• 出版日期2017-6
• 单位新乡医学院