Axial Nanometer Distances Measured by Fluorescence Lifetime Imaging Microscopy

作者：Berndt Michael; Lorenz Mike; Enderlein Joerg; Diez Stefan^*

来源：Nano Letters, 2010, 10(4): 1497-1500.

DOI：10.1021/nl100593x

• Absolute distance measurement
• fluorescence lifetime
• FLIM
• microtubules
• avidin
• neutravidin
• kinesin-1

We present a novel fluorescence lifetime imaging microscopy technique to measure absolute positions of fluorescent molecules within 100 nm above a metalized surface based on distance-dependent fluorescence lifetime modulations. We apply this technique to fluorescently labeled microtubules as optical probes with various unlabeled proteins attached. By measuring the fluorescence lifetimes, we obtain the position of the microtubules and therefore determine the geometrical size of the attached proteins with nanometer precision.

• 出版日期2010-4