A Multicommuted Flow Analysis Method for the Photometric Determination of Amoxicillin in Pharmaceutical Formulations using a Diazo Coupling Reaction

作者：Freitas Sueny K B; da Silva Valdinete Lins; Araujo Alberto N; Montenegro Maria Conceicao B S M; Reis Boaventura F; Paim Ana Paula S^*

来源：Journal of the Brazilian Chemical Society, 2011, 22(2): 279-285.

• amoxicillin
• multicommuted flow analysis
• photometry
• diazo coupling reaction
• o-nitroaniline

A multicommuted flow analysis method for the spectrophotometric determination of amoxicillin in pharmaceutical formulations is reported. The proposed method is based on the reaction of diazotised o-nitroaniline with amoxicillin in an alkaline medium, producing a yellow compound that was monitored at 435 nm. The main features of the method were: a linear relationship with amoxicillin concentration ranging from 25 to 400 mg L(-1); a detection limit of 5.1 mg L(-1); an analytical throughput of 50 determinations per hour; and a relative standard deviation lower than 3.9% for a typical amoxicillin sample with concentration of 100 mg L(-1) (n = 10). To verify the accuracy of the method, samples were also analyzed employing an independent method, and applying the paired t-est to the results no significant difference at the 95% confidence level was observed.

• 出版日期2011-2